Imagerie pour la biologie et la santé (IBS)

Imagerie optique plein champ d'un explant de rétine de souris

Responsable

Emmanuel FORT

Tél. : 01 80 96 30 35

Co-responsable

Ignacio IZEDDIN

Tél. : 01 80 96 30 75

Membres permanents

Michael ATLAN

Tél. : 01 80 96 30 36

Pedro BARAÇAL DE MECÊ

Tél. : 01 80 96 30 51

Claude BOCCARA

Tél. : 01 80 96 30 44

Maïmouna BOCOUM

Tél. : 01 80 96 30 76

Clément CABRIEL

Tél. : 01 80 96 30 59

Bruce DENBY

Emmanuel FORT

Tél. : 01 80 96 30 35

Ros-Kiri ING

Tél. : 01 80 96 30 41

Ignacio IZEDDIN

Tél. : 01 80 96 30 75

François RAMAZ

Tél. : 01 80 96 30 47

Olivier THOUVENIN

Tél. : 01 80 96 30 51

Actualités

Sensitivity of Lamb waves in viscoelastic polymer plates to surface contamination Spytek, J., D. A. Kiefer, R. K. Ing, C. Prada, J. Grando, and J. De Rosny Ultrasonics 149, 107571 (2025) Résumé: Detecting surface contamination on thin thermoformed polymer plates is a critical issue for various industrial applications. Lamb waves offer a promising solution, though their effectiveness is challenged by the strong attenuation and anisotropy of the polymer plates. This issue is addressed in the context of a calcium carbonate (CaCO3) layer deposited on a polypropylene (PP) plate. First, the viscoelastic properties of the PP material are determined using a genetic algorithm inversion of data measured with a scanning laser vibrometer. Second, using a bi-layer plate model, the elastic properties and thickness of the CaCO3 layer are estimated. Based on the model, the sensitivity analysis is performed, demonstrating considerable effectiveness of the A1 Lamb mode in detecting thin layers of CaCO3 compared to Lamb modes A0 and S0. Finally, a direct application of this work is illustrated through in-situ monitoring of CaCO3 contaminants using a straightforward inter-transducer measurement.
Submacular Choroidal Arteries: A Laser Doppler Holography and OCT Study Paques, M., Z. Bratasz, L. Puyo, C. Chaumette, D. Castro Farias, M. Atlan, and S. Mrejen Ophthalmology Science 5, no. 3, 100709 (2025) Résumé: Objective: To document the aspect, topography and morphometry of normal human choroidal arteries in the posterior pole by laser Doppler holography (LDH) and OCT. Design: Cross-sectional study. Subjects: Fifty-four eyes of 27 healthy subjects. Methods: A prototypic LDH system captured the laser Doppler shift of the choroidal circulation within the central 20°. Doppler shifts were filtered to extract high velocity vessels. Images of choroidal arteries identified by LDH were subsequently registered with en face and cross-sectional OCT images. Subsequently, the diameters of macular choroidal arteries and their correlation to central choroidal thickness was measured on OCT B-scans. Main Outcome Measures: Spatial disposition, distribution, and diameters of choroidal arteries. Results: Choroidal arteries were identified by LDH and OCT from their emergence from short posterior ciliary arteries (sPCAs), and could be traced to second and third divisions. In the 8 eyes that underwent LDH, 7 of 8 (88%) showed a horizontal first-order artery within 0.5 disc diameter from the fovea. OCT B-scans showed that first-order arteries were located along the sclera-choroid interface; around arteries, the choroidal tissue formed a pyramid-shaped avascular structure with a posterior base contiguous and isoreflective to the sclera. In a cohort of 49 eyes, the diameter of horizontal submacular arteries (average [± standard deviation] 136.3 μm [±47]; range, 70–209 μm) was weakly correlated to central choroidal thickness (P = 0.09). Conclusions: First-order choroidal arteries emerging from sPCAs are located along the sclerochoroidal interface and are surrounded by a pyramid-shaped avascular space, which contributes to differentiate them from veins. The majority of normal eye show a submacular first-order artery running horizontally toward the temporal periphery. These results will pave the way for a better knowledge of diseases affecting the choroidal circulation. Financial Disclosure(s): Proprietary or commercial disclosure may be found in the Footnotes and Disclosures at the end of this article.
3D Single-Molecule Super-Resolution Imaging of Microfabricated Multiscale Fractal Substrates for Calibration and Cell Imaging Cabriel, C., R. M. Córdova-Castro, E. Berenschot, A. Dávila-Lezama, K. Pondman, S. Le Gac, N. Tas, A. Susarrey-Arce, and I. Izeddin ACS Applied Materials and Interfaces 17, no. 6, 9019-9034 (2025) Résumé: Microstructures arrayed over a substrate have shown increasing interest due to their ability to provide advanced 3D cellular models, which open up new possibilities for cell culture, proliferation, and differentiation. Still, the mechanisms by which physical cues impact the cell phenotype are not fully understood, hence the necessity to interrogate cell behavior at the highest resolution. However, cell 3D high-resolution optical imaging on such microstructured substrates remains challenging due to their complexity as well as axial calibration issues. In this work, we address this issue by leveraging the geometrical characteristics of fractal-like structures, which serve as axial calibration tools and modulate cell growth. To this end, we use multiscale 3D SiO2 substrates consisting of spatially arrayed octahedral features of a few micrometers to hundreds of nanometers. Through optimizations of both the structures and optical imaging conditions, we demonstrate the potential of these 3D multiscale structures as an alternative to electron microscopy for material imaging but also as calibration tools for 3D super-resolution microscopy. We used their multiscale and known geometry to perform lateral and axial calibrations in 3D single-molecule localization microscopy (SMLM) and assess imaging resolutions. We then utilized these substrates as a platform for high-resolution bioimaging. As a proof of concept, we cultivate human mesenchymal stem cells on these substrates, revealing very different growth patterns compared to flat glass. Specifically, the spatial distribution of cytoskeleton proteins is vastly modified, as we demonstrate with a 3D SMLM assessment. Mots-clés: 3D single-molecule localization microscopy; bioimaging; multiscale material; fractal-like microstructures; calibration; material imaging
Rapid On-Site Histopathological Analysis of Kidney Biopsy With Dynamic Full-Field Optical Coherence Tomography Zuccarelli, L., Q. Bernard, G. Tarris, L. Martin, M. Funes De La Vega, A. Jacq, J. M. Rebibou, C. Tinel, C. Boccara, O. Thouvenin, J. M. Chassot, M. Rabant, J. Zuber, C. Legendre, J. P. Quenot, M. N. Peraldi, L. Amrouche, A. Scemla, N. Chavarot, D. Anglicheau, M. Legendre, and T. Maldiney Kidney International Reports (2025) Résumé: Introduction: Kidney histology preparation requires a multistep process that is usually responsible for delayed results. This study introduces dynamic full-field optical coherence tomography (D-FF-OCT) as a label-free alternative to overcome the limitations of traditional histopathology for on-site kidney pathology assessment. Methods: Two patient cohorts were considered, with a total of 31 patients included in the study; one cohort involved patients requiring biopsy of transplant kidney, and the other involved patients requiring biopsy of native kidney. The clinical and biological data were prospectively collected. Histopathological analysis of kidney biopsies was conducted using both conventional stains and dynamic D-FF-OCT imaging. Results: D-FF-OCT enabled the recognition of most kidney structures. The results showed a significant correlation between this technology and conventional stains for the evaluation of both interstitial fibrosis (IF) (r = 0.61, P < 0.001) and tubular atrophy (TA) (r = 0.60, P < 0.001). Although many lesions could be identified such as interstitial inflammation, acute tubular necrosis, glomerular crescents, and vascular intimal thickening; other recognitions such as glomerular membranous deposits, vascular amyloidosis, and peritubular capillaritis will require confirmation in larger cohorts. Conclusion: This study demonstrates the potential of D-FF-OCT imaging for on-site analysis of kidney biopsies, providing rapid and high-resolution images without extensive sample preparation.

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Présentation du thème

Le thème IBS étudie les processus et tissus biologiques de l’échelle nanoscopique à macroscopique. Via l’étude approfondie de l’interaction entre différentes ondes et la matière biologique, de nouvelles méthodes d’imagerie non conventionnelles sont développées. L’imagerie de super-résolution permettant de sonder l’organisation de la matière à l’échelle de la molécule individuelle et de comprendre le transport de ces molécules dans l’environnement de la cellule et en particulier dans le noyau. L’équipe imagerie optique plein champ s’intéresse à l’organisation des tissus biologiques par des techniques de microscopie interférométrique sans marquage, et permettant d’étudier la viabilité des cellules et tissus d’un organisme via le suivi des organelles cellulaires, pour des applications tant de biologie fondamentale que de diagnostic médical in vivo. L’équipe imagerie Doppler holographique de la rétine est spécialisée dans la quantification des flux sanguins dans la rétine de patients humains. Néanmoins, les approches optiques présentées jusqu’à là sont limitées à des faibles profondeurs de pénétration dans le corps humain (en dehors de l’œil transparent). Afin de pouvoir sonder les tissus biologiques plus en profondeur, il devient nécessaire d’utiliser d’autres types d’ondes, en particulier les ondes acoustiques. L’imagerie acousto-optique permet d’obtenir des images de tissus avec un contraste optique à plusieurs millimètres de profondeur en marquant les photons multiplement diffusés grâce aux ondes acoustiques. Finalement, l’utilisation d’ondes acoustiques permet à l’équipe Mouvements de surface de cartographier les déformations vibratoires du thorax sans contact et de manière non invasive.

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